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ultra ii end prep reaction buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs ultra ii end prep reaction buffer
    Ultra Ii End Prep Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1871 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ultra+ii+end+prep+reaction+buffer/NEBNext+Ultra+II+End+Repair%2FdA-Tailing+M/med_rxiv__64898__2026__04__08__26350410-181-16-21
    Average 99 stars, based on 1871 article reviews
    ultra ii end prep reaction buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Formalin-fixed Paraffin-Embedded:

    Article Title: CRISPR/Cas9-mediated generation of a de novo C9ORF72 knock-in isogenic iPSC cell bank to model ALS and FTD
    Article Snippet: .. For library preparation 1-1.5 μg of sample in 48 μL of nuclease-free water (NFW) was mixed with 3.5 μL NEBNext FFPE DNA Repair Buffer, 3.5 μL Ultra II End-Prep Reaction Buffer, 2 μL NEBNext FFPE DNA Repair Mix (M6630, NEB) and 3 μL Ultra II End-Prep Enzyme Mix (E7546, NEB) and incubated at 20°C for 10 minutes followed by 65°C for 10 minutes. .. The reaction was then incubated at room temperature for 10 minutes on a hula mixer with 60 μL of AMPure XP Beads (A63881, Beckman Coulter).

    Article Title: Single-molecule cfDNA sequencing establishes clinical utility for ecDNA monitoring and multimodal liquid biopsy analysis
    Article Snippet: .. Following cfDNA extraction, DNA was end-repaired by mixing 0.875μl of NEBNext FFPE DNA Repair Buffer, 0.875μl Ultra II End-Prep Reaction buffer (NEB, Cat. No.: E7546 component E7647AVIAL), 0.75μl Ultra II End-Prep Enzyme Mix (NEB, Cat. No.:E7546 component E7646AVIAL) and 0.5μl NEBNext FFPE DNA Repair Mix (NEB, Cat. No.: M6630) with 12μl of extracted cfDNA. ..

    Article Title: A multimodal screening platform for endogenous dipeptide repeat proteins in C9orf72 patient iPSC neurons.
    Article Snippet: .. For library preparation 1–1.5 μg of sample in 48 μL of nuclease-free water (NFW) was mixed with 3.5 μL NEBNext FFPE DNA Repair Buffer, 3.5 μL Ultra II End-Prep Reaction Buffer, 2 μL NEBNext FFPE DNA Repair Mix (M6630, NEB) and 3 μL Ultra II End-Prep Enzyme Mix (E7546, NEB) and incubated at 20◦C for 10 min followed by 65◦C for 10 min. .. The reaction was then incubated at room temperature for 10 min on a hula mixer with 60 μL of AMPure XP Beads (A63881, Beckman Coulter).

    Article Title: High-throughput nanopore DNA sequencing of large insert fosmid clones directly from bacterial colonies
    Article Snippet: .. phi29-XT and phi29-XT reaction buffer (from E1603: phi29-XT RCA Kit), dNTP solution mix, SacI-HF, XhoI, rCutSmart buffer, T7 endonuclease I, NEBuffer 2, NEBNext FFPE DNA repair mix, NEBNext FFPE DNA repair buffer, NEBNext Ultra II end prep enzyme mix, Next Ultra II end prep reaction buffer, Blunt/TA ligase master mix, NEBNext Quick ligation reaction buffer, Quick T4 DNA ligase were all from New England Biolabs (Ipswich, MA, USA). .. UltraPure bovine serum albumin (BSA) was from Invitrogen (Waltham, MA, USA).

    Article Title: Generation of C9orf72 h370 mice, an intron 1 humanised C9orf72 repeat-expansion knock-in model
    Article Snippet: .. For library preparation 1-1.5 μg of sample in 48 μl of nuclease-free water (NFW) was mixed with 3.5 μl NEBNext FFPE DNA Repair Buffer, 3.5 μl Ultra II End-Prep Reaction Buffer, 2 μl NEBNext FFPE DNA Repair Mix (M6630, NEB) and 3 μl Ultra II End-Prep Enzyme Mix (E7546, NEB) and incubated at 20°C for 10 minutes followed by 65°C for 10 minutes. .. The reaction was then incubated at room temperature for 10 minutes on a hula mixer with 60 μl of AMPure XP Beads (A63881, Beckman Coulter).

    Incubation:

    Article Title: CRISPR/Cas9-mediated generation of a de novo C9ORF72 knock-in isogenic iPSC cell bank to model ALS and FTD
    Article Snippet: .. For library preparation 1-1.5 μg of sample in 48 μL of nuclease-free water (NFW) was mixed with 3.5 μL NEBNext FFPE DNA Repair Buffer, 3.5 μL Ultra II End-Prep Reaction Buffer, 2 μL NEBNext FFPE DNA Repair Mix (M6630, NEB) and 3 μL Ultra II End-Prep Enzyme Mix (E7546, NEB) and incubated at 20°C for 10 minutes followed by 65°C for 10 minutes. .. The reaction was then incubated at room temperature for 10 minutes on a hula mixer with 60 μL of AMPure XP Beads (A63881, Beckman Coulter).

    Article Title: A multimodal screening platform for endogenous dipeptide repeat proteins in C9orf72 patient iPSC neurons.
    Article Snippet: .. For library preparation 1–1.5 μg of sample in 48 μL of nuclease-free water (NFW) was mixed with 3.5 μL NEBNext FFPE DNA Repair Buffer, 3.5 μL Ultra II End-Prep Reaction Buffer, 2 μL NEBNext FFPE DNA Repair Mix (M6630, NEB) and 3 μL Ultra II End-Prep Enzyme Mix (E7546, NEB) and incubated at 20◦C for 10 min followed by 65◦C for 10 min. .. The reaction was then incubated at room temperature for 10 min on a hula mixer with 60 μL of AMPure XP Beads (A63881, Beckman Coulter).

    Article Title: Generation of C9orf72 h370 mice, an intron 1 humanised C9orf72 repeat-expansion knock-in model
    Article Snippet: .. For library preparation 1-1.5 μg of sample in 48 μl of nuclease-free water (NFW) was mixed with 3.5 μl NEBNext FFPE DNA Repair Buffer, 3.5 μl Ultra II End-Prep Reaction Buffer, 2 μl NEBNext FFPE DNA Repair Mix (M6630, NEB) and 3 μl Ultra II End-Prep Enzyme Mix (E7546, NEB) and incubated at 20°C for 10 minutes followed by 65°C for 10 minutes. .. The reaction was then incubated at room temperature for 10 minutes on a hula mixer with 60 μl of AMPure XP Beads (A63881, Beckman Coulter).

    Extraction:

    Article Title: Single-molecule cfDNA sequencing establishes clinical utility for ecDNA monitoring and multimodal liquid biopsy analysis
    Article Snippet: .. Following cfDNA extraction, DNA was end-repaired by mixing 0.875μl of NEBNext FFPE DNA Repair Buffer, 0.875μl Ultra II End-Prep Reaction buffer (NEB, Cat. No.: E7546 component E7647AVIAL), 0.75μl Ultra II End-Prep Enzyme Mix (NEB, Cat. No.:E7546 component E7646AVIAL) and 0.5μl NEBNext FFPE DNA Repair Mix (NEB, Cat. No.: M6630) with 12μl of extracted cfDNA. ..

    Polymerase Chain Reaction:

    Article Title: High-performance protocol for ultra-short DNA sequencing using Oxford Nanopore Technology (ONT)
    Article Snippet: .. Materials 0.2 mL PCR tube 1.5 mL RNase- and DNase-free low-bind tube Milli-Q water (Thermo Fisher Scienti c) Ultra II End-Prep Reaction Buffer (E7546S, NEB) Ultra II End-Prep Enzyme Mix (E7546S, NEB) Ligation Buffer (LNB, ONT) Quick T4 DNA Ligase (E6056S, NEB) Ligation Adapters (LA, ONT) Small Fragment Buffer (SFB, ONT) Elution Buffer (EB, ONT) Qubit HS dsDNA kit (Thermo Fisher Scienti c) Flongle (ONT) ow cell MinION Mk1B device (ONT) Flow Cell Flush (FCF, ONT) Flow Cell Tether (FCT, ONT) Sequencing Buffer (SB, ONT) Library Beads (LIB, ONT) NEBNext Ultra II End Repair/dA-Tailing Module - 24 rxns New England Biolabs Catalog #E7546S NEBNext Quick Ligation Module New England Biolabs Catalog #E6056S protocols.io | https://dx.doi.org/10.17504/protocols.io.dm6gp973jvzp/v1 February 26, 2025 2/7 Before start Thaw all the reagents and keep them on ice throughout the protocol. ..

    Ligation:

    Article Title: High-performance protocol for ultra-short DNA sequencing using Oxford Nanopore Technology (ONT)
    Article Snippet: .. Materials 0.2 mL PCR tube 1.5 mL RNase- and DNase-free low-bind tube Milli-Q water (Thermo Fisher Scienti c) Ultra II End-Prep Reaction Buffer (E7546S, NEB) Ultra II End-Prep Enzyme Mix (E7546S, NEB) Ligation Buffer (LNB, ONT) Quick T4 DNA Ligase (E6056S, NEB) Ligation Adapters (LA, ONT) Small Fragment Buffer (SFB, ONT) Elution Buffer (EB, ONT) Qubit HS dsDNA kit (Thermo Fisher Scienti c) Flongle (ONT) ow cell MinION Mk1B device (ONT) Flow Cell Flush (FCF, ONT) Flow Cell Tether (FCT, ONT) Sequencing Buffer (SB, ONT) Library Beads (LIB, ONT) NEBNext Ultra II End Repair/dA-Tailing Module - 24 rxns New England Biolabs Catalog #E7546S NEBNext Quick Ligation Module New England Biolabs Catalog #E6056S protocols.io | https://dx.doi.org/10.17504/protocols.io.dm6gp973jvzp/v1 February 26, 2025 2/7 Before start Thaw all the reagents and keep them on ice throughout the protocol. ..

    Article Title: High-throughput nanopore DNA sequencing of large insert fosmid clones directly from bacterial colonies
    Article Snippet: .. phi29-XT and phi29-XT reaction buffer (from E1603: phi29-XT RCA Kit), dNTP solution mix, SacI-HF, XhoI, rCutSmart buffer, T7 endonuclease I, NEBuffer 2, NEBNext FFPE DNA repair mix, NEBNext FFPE DNA repair buffer, NEBNext Ultra II end prep enzyme mix, Next Ultra II end prep reaction buffer, Blunt/TA ligase master mix, NEBNext Quick ligation reaction buffer, Quick T4 DNA ligase were all from New England Biolabs (Ipswich, MA, USA). .. UltraPure bovine serum albumin (BSA) was from Invitrogen (Waltham, MA, USA).

    Sequencing:

    Article Title: High-performance protocol for ultra-short DNA sequencing using Oxford Nanopore Technology (ONT)
    Article Snippet: .. Materials 0.2 mL PCR tube 1.5 mL RNase- and DNase-free low-bind tube Milli-Q water (Thermo Fisher Scienti c) Ultra II End-Prep Reaction Buffer (E7546S, NEB) Ultra II End-Prep Enzyme Mix (E7546S, NEB) Ligation Buffer (LNB, ONT) Quick T4 DNA Ligase (E6056S, NEB) Ligation Adapters (LA, ONT) Small Fragment Buffer (SFB, ONT) Elution Buffer (EB, ONT) Qubit HS dsDNA kit (Thermo Fisher Scienti c) Flongle (ONT) ow cell MinION Mk1B device (ONT) Flow Cell Flush (FCF, ONT) Flow Cell Tether (FCT, ONT) Sequencing Buffer (SB, ONT) Library Beads (LIB, ONT) NEBNext Ultra II End Repair/dA-Tailing Module - 24 rxns New England Biolabs Catalog #E7546S NEBNext Quick Ligation Module New England Biolabs Catalog #E6056S protocols.io | https://dx.doi.org/10.17504/protocols.io.dm6gp973jvzp/v1 February 26, 2025 2/7 Before start Thaw all the reagents and keep them on ice throughout the protocol. ..



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